Israeli defence minister has warned the Syrian government that it would pay a "high price" for helping armed groups bent on attacking his country, Al Jazeera reported.
Moshe Yaalon comments on Wednesday came a day after roadside bombing, blamed on Damascus, wounded four Israeli troops in the Israeli-occupied Golan Heights near the ceasefire line with Syria.
Israeli air force responded 12 hours later by bombing Syrian military targets.
"We see [the President Bashar] al-Assad regime as responsible for what is happening under its authority, and if it continues to cooperate with terror elements who seek to harm Israel, we will make it pay a high price," Yaalon said.
Syria's forces are supported in their battle against rebels seeking to topple Assad by fighters from Hezbollah, a powerful Lebanese Shia movement which battled Israel to a bloody stalemate in a 2006 war.
"We will not put up with any breach of our sovereignty or attack on our soldiers or citizens, and we will react with determination and force against anyone acting against us, at any time and any place, as we have done overnight," Yaalon said in a statement.
"Whoever tries to harm us takes his fate in his hands."