The turnout was revealed with just hours to go until the polls open. There are a total of 377,413 people registered to take part in the city, Edinburgh News reported.
Sue Bruce, Counting Officer for Edinburgh and Chief Executive of City of Edinburgh Council, said: "After months of anticipation, the Referendum is finally upon us, and hundreds of thousands of people across the city are about to cast their vote.
"I am really pleased with the high rate of registration, which demonstrates the level of interest in the debate. We have a record number of registered voters in Edinburgh for this Referendum and therefore I would encourage visitors to leave plenty of time to visit their local polling station and to mark an X in the box of their choice when filling out their ballot papers."
Polling places will be open between 7am and 10pm on Thursday, though the public are being advised to avoid busy periods, expected to be before and after normal working hours. Where possible, voters are encouraged to visit mid-morning or mid-afternoon.
Information on local polling places can be found on the polling card delivered to homes or on the Council website.